Imaging:Article Title: PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate
Article Snippet: .. For organoid culture maintenance and imaging, cells derived from dorsolateral prostate (DLP) lobes were cultured by overlaying 10000 cells, in 400 μl full organoid growth medium (DMEM/F12 or ADMEM/F12 supplemented with 10 mM HEPES pH 7.4, 2 mM Glutamax and 1x Penicillin Streptomycin, as well as additional components B27 minus vitamin A, N-acetyl-L-cysteine (1.25 mM), murine EGF (50 ng/ml), recombinant murine Noggin (100 ng/ml), recombinant human R-Spondin-1 (500 ng/ml), Y-27632 dihydrochloride (10 μM), 5α-Dihydrotestosterone (DHT) solution (1 nM) and A 83-01 (200 nM), onto 95 μl Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, in a Nunc LabTek II chamber slide system (4 well; glass). ..
Derivative Assay:Article Title: PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate
Article Snippet: .. For organoid culture maintenance and imaging, cells derived from dorsolateral prostate (DLP) lobes were cultured by overlaying 10000 cells, in 400 μl full organoid growth medium (DMEM/F12 or ADMEM/F12 supplemented with 10 mM HEPES pH 7.4, 2 mM Glutamax and 1x Penicillin Streptomycin, as well as additional components B27 minus vitamin A, N-acetyl-L-cysteine (1.25 mM), murine EGF (50 ng/ml), recombinant murine Noggin (100 ng/ml), recombinant human R-Spondin-1 (500 ng/ml), Y-27632 dihydrochloride (10 μM), 5α-Dihydrotestosterone (DHT) solution (1 nM) and A 83-01 (200 nM), onto 95 μl Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, in a Nunc LabTek II chamber slide system (4 well; glass). ..
Article Title: PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate.
Article Snippet: .. Organoid culture Prostate cell isolation and subsequent organoid culture was performed according to methodology described by Karthaus et al.84 For organoid culturemaintenance, cells derived from dorsolateral prostate (DLP) lobes were cultured by overlaying 10000 cells, in 400 mL full organoid growth medium (ADMEM/F12 supplemented with 10 mM HEPES pH 7.4, 2 mM Glutamax and 1x PenicillinStreptomycin, as well as additional components B27 minus vitamin A, N-acetyl-L-cysteine (1.25 mM), murine EGF (50 ng/mL), recombinant murine Noggin (100 ng/mL), recombinant human R-Spondin-1 (500 ng/mL), Y-27632 dihydrochloride (10 mM), 5a-Dihydrotestosterone (DHT) solution (1 nM) and A 83-01 (200 nM), onto 95 mL Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, in a Nunc LabTek II chamber slide system (4-well; glass). ..
Cell Culture:Article Title: PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate
Article Snippet: .. For organoid culture maintenance and imaging, cells derived from dorsolateral prostate (DLP) lobes were cultured by overlaying 10000 cells, in 400 μl full organoid growth medium (DMEM/F12 or ADMEM/F12 supplemented with 10 mM HEPES pH 7.4, 2 mM Glutamax and 1x Penicillin Streptomycin, as well as additional components B27 minus vitamin A, N-acetyl-L-cysteine (1.25 mM), murine EGF (50 ng/ml), recombinant murine Noggin (100 ng/ml), recombinant human R-Spondin-1 (500 ng/ml), Y-27632 dihydrochloride (10 μM), 5α-Dihydrotestosterone (DHT) solution (1 nM) and A 83-01 (200 nM), onto 95 μl Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, in a Nunc LabTek II chamber slide system (4 well; glass). ..
Article Title: PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate.
Article Snippet: .. Organoid culture Prostate cell isolation and subsequent organoid culture was performed according to methodology described by Karthaus et al.84 For organoid culturemaintenance, cells derived from dorsolateral prostate (DLP) lobes were cultured by overlaying 10000 cells, in 400 mL full organoid growth medium (ADMEM/F12 supplemented with 10 mM HEPES pH 7.4, 2 mM Glutamax and 1x PenicillinStreptomycin, as well as additional components B27 minus vitamin A, N-acetyl-L-cysteine (1.25 mM), murine EGF (50 ng/mL), recombinant murine Noggin (100 ng/mL), recombinant human R-Spondin-1 (500 ng/mL), Y-27632 dihydrochloride (10 mM), 5a-Dihydrotestosterone (DHT) solution (1 nM) and A 83-01 (200 nM), onto 95 mL Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, in a Nunc LabTek II chamber slide system (4-well; glass). ..
Recombinant:Article Title: PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate
Article Snippet: .. For organoid culture maintenance and imaging, cells derived from dorsolateral prostate (DLP) lobes were cultured by overlaying 10000 cells, in 400 μl full organoid growth medium (DMEM/F12 or ADMEM/F12 supplemented with 10 mM HEPES pH 7.4, 2 mM Glutamax and 1x Penicillin Streptomycin, as well as additional components B27 minus vitamin A, N-acetyl-L-cysteine (1.25 mM), murine EGF (50 ng/ml), recombinant murine Noggin (100 ng/ml), recombinant human R-Spondin-1 (500 ng/ml), Y-27632 dihydrochloride (10 μM), 5α-Dihydrotestosterone (DHT) solution (1 nM) and A 83-01 (200 nM), onto 95 μl Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, in a Nunc LabTek II chamber slide system (4 well; glass). ..
Article Title: PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate.
Article Snippet: .. Organoid culture Prostate cell isolation and subsequent organoid culture was performed according to methodology described by Karthaus et al.84 For organoid culturemaintenance, cells derived from dorsolateral prostate (DLP) lobes were cultured by overlaying 10000 cells, in 400 mL full organoid growth medium (ADMEM/F12 supplemented with 10 mM HEPES pH 7.4, 2 mM Glutamax and 1x PenicillinStreptomycin, as well as additional components B27 minus vitamin A, N-acetyl-L-cysteine (1.25 mM), murine EGF (50 ng/mL), recombinant murine Noggin (100 ng/mL), recombinant human R-Spondin-1 (500 ng/mL), Y-27632 dihydrochloride (10 mM), 5a-Dihydrotestosterone (DHT) solution (1 nM) and A 83-01 (200 nM), onto 95 mL Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, in a Nunc LabTek II chamber slide system (4-well; glass). ..
Membrane:Article Title: PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate
Article Snippet: .. For organoid culture maintenance and imaging, cells derived from dorsolateral prostate (DLP) lobes were cultured by overlaying 10000 cells, in 400 μl full organoid growth medium (DMEM/F12 or ADMEM/F12 supplemented with 10 mM HEPES pH 7.4, 2 mM Glutamax and 1x Penicillin Streptomycin, as well as additional components B27 minus vitamin A, N-acetyl-L-cysteine (1.25 mM), murine EGF (50 ng/ml), recombinant murine Noggin (100 ng/ml), recombinant human R-Spondin-1 (500 ng/ml), Y-27632 dihydrochloride (10 μM), 5α-Dihydrotestosterone (DHT) solution (1 nM) and A 83-01 (200 nM), onto 95 μl Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, in a Nunc LabTek II chamber slide system (4 well; glass). ..
Article Title: PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate.
Article Snippet: .. Organoid culture Prostate cell isolation and subsequent organoid culture was performed according to methodology described by Karthaus et al.84 For organoid culturemaintenance, cells derived from dorsolateral prostate (DLP) lobes were cultured by overlaying 10000 cells, in 400 mL full organoid growth medium (ADMEM/F12 supplemented with 10 mM HEPES pH 7.4, 2 mM Glutamax and 1x PenicillinStreptomycin, as well as additional components B27 minus vitamin A, N-acetyl-L-cysteine (1.25 mM), murine EGF (50 ng/mL), recombinant murine Noggin (100 ng/mL), recombinant human R-Spondin-1 (500 ng/mL), Y-27632 dihydrochloride (10 mM), 5a-Dihydrotestosterone (DHT) solution (1 nM) and A 83-01 (200 nM), onto 95 mL Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, in a Nunc LabTek II chamber slide system (4-well; glass). ..
Cell Isolation:Article Title: PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate.
Article Snippet: .. Organoid culture Prostate cell isolation and subsequent organoid culture was performed according to methodology described by Karthaus et al.84 For organoid culturemaintenance, cells derived from dorsolateral prostate (DLP) lobes were cultured by overlaying 10000 cells, in 400 mL full organoid growth medium (ADMEM/F12 supplemented with 10 mM HEPES pH 7.4, 2 mM Glutamax and 1x PenicillinStreptomycin, as well as additional components B27 minus vitamin A, N-acetyl-L-cysteine (1.25 mM), murine EGF (50 ng/mL), recombinant murine Noggin (100 ng/mL), recombinant human R-Spondin-1 (500 ng/mL), Y-27632 dihydrochloride (10 mM), 5a-Dihydrotestosterone (DHT) solution (1 nM) and A 83-01 (200 nM), onto 95 mL Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, in a Nunc LabTek II chamber slide system (4-well; glass). ..
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